This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a ?fingerprint? for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of F?rster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Advanced Photon Counting Ebook
Applications, Methods, Instrumentation
By: Peter Kapusta
Publisher:
Springer
Print ISBN: 9783319156354, 3319156357
eText ISBN: 9783319156361, 3319156365
Copyright year: 2015
Format: PDF
Available from $ 289.00 USD
SKU: 9783319156361
No Sampling
Download immediately after payment, copy on your device does not expire, Download link will send to your email and backup on your account.
